A position is available in the laboratory of T. Melendy, in the Departments of Microbiology and Biochemistry at the UB Medical School, to investigate how medically relevant small DNA viruses (HPV, JC and BK) hijack the cellular DNA replication machinery to replicate viral genomes; our studies are being leveraged to identify small molecules for development of anti-viral treatments. We use molecular biology, biochemistry, and cell biology approaches to elucidate how the viral DNA replication proteins interact with cellular proteins, and how the activities of these enzymes are modulated by protein-protein interaction and post-translational modifications. Current approaches in use include: biochemical in vitro assays, cell transfection/luciferase assays, high throughput assays for small molecule and point mutation screening (based on bioinformatic and structural bioinformatic analyses), and immuno-staining of transfected cells. We currently have six manuscripts in preparation for submission, and expect more next year. Currently, there are projects available for rapid completion by a proactive applicant. A successful applicant should be highly skilled in sub-cloning, protein expression and purification (at least via affinity tags), and human cell culture and transfection. Experience with immuno-staining and baculovirus expression is also desirable. This position is immediately available, funded by an existing NIH grant, with full fringe benefits.
Ph.D. degree in Microbiology, Biochemistry or related subject required
Previous experience with immuno-staining of culture human cells, baculovirus expression, and preparing samples for phospho-MS analyses are preferred. A successful applicant should be highly skilled in sub-cloning, protein expression and purification, and human cell culture and transfection
Contact’s Name Michelle Bice
Contact’s Title Grant Manager
Contact’s Email firstname.lastname@example.org
Contact’s Phone 716-829-5424