Bacteria have a high level of internal organization, with a subset of proteins and protein complexes having specific subcellular addresses. Examples of subcellular organization can be seen in chromosomal segregation, appendage localization (such as type IV pili and flagella), and division. Our research, which focuses on the mechanisms used to create and maintain this internal organization, will further increase the understanding of the bacterial physiology and lifecycle. We are investigating membrane proteins of Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen that can infect burn victims and those with cystic fibrosis. P. aeruginosa is also a ubiquitous organism, found widely throughout nature, and as such is very versatile. Using proteomics in combination with mutant strains of P. aeruginosa our lab is identifying and characterizing novel polar and septal membrane proteins. These novel proteins will then be used to study the mechanisms for achieving and maintaining the spatial organization within the bacterial cell.
