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Job ID: 206951

Scientist III - Chemistry
Tufts University

Date Posted Mar. 9, 2023
Title Scientist III - Chemistry
University Tufts University
Medford, MA, United States
Department School of Arts and Sciences
Application Deadline Open until filled
Position Start Date Available immediately
  • Research Scientist/Associate
  • Chemistry - General
    Biology - General

What You'll Do

A Research Scientist drives a research program that directly contributes to the mission. Working independently, but in close consultation and in cooperation with the [INSERT ROLE] at Tufts University, this individual must be able to:

  • Develop, adapt, and implement new research techniques and protocols.
  • Analyzes, interprets, summarizes and compiles data. Perform routine and complex laboratory procedures.
  • Collaborate on current/future grants through participation in grant proposals, submissions, publications and presentations.
  • Participate in contributing data to publications and presentations as co-author.
  • Participate in teaching, research, and mentoring including providing research guidance to graduate students or postdoctoral scholars.

What We're Looking For

Basic Requirements:

  • A PhD and at least one year of post-doctoral training is required for this position.
  • The individual must have demonstrated that he/she is able to make significant contributions to related scientific research and scholarship (publication in good journals)
  • Have potential to direct his/her own research
  • They will be expected to publish regular, significant contributions in peer-reviewed journals.

Preferred Qualifications:

  • Demonstrated expertise in executing target identification strategies including the design and execution of mass-spectrometry based interaction proteomics/metabolomics workflows and validation of targets via functional genetic screens (eg. CRISPR-Cas9, shRNA).
  • Experience in protein isolation from cells, tissues and biofluids, sub-cellular fractionation, immuno- or chemical affinity enrichment to selectively enrich proteins from complex biological systems using IP-MS, AP-MS and proximity labeling assays.
  • Proficient in developing and utilizing cell models to enable the discovery of novel biology, answer mechanistic questions, and validate potential targets via stable cell line generation and maintenance (lentiviral transduction) in BSL2 laboratory settings.
  • Expertise in quantitative mass spectrometry-based proteomics and data analysis pipelines for in-vitro and ex-vivo quantitative proteomics applications. 
  • Proficiency using Thermofisher Orbitrap mass spectrometers and nanoLC's: Orbitrap Exploris and Vanquish Neo systems. 
  • Expertise in multi-dimensional LC fractionation and high-throughput quantitative proteomics methodologies such as TMT®, Label-free quantification and targeted (SRM and PRM) acquisition.
  • Expertise in mass spectrometric data analysis using Xcalibur™, Proteome Discoverer™, MaxQuant, Skyline, and other DB search engine tools.
  • Strong knowledge of biostatistics and omics data analysis.
  • Expertise in making BSL-2 pseudo-viral particles for experimental infection assays.
  • Expertise in designing and executing immunostaining assays and confocal image analysis.
  • Experience with handling, processing and analyzing various samples, such as cell lines, plasma, serum, urine and tissues.

Pay Range

Minimum $62,200.00, Midpoint $77,750.00, Maximum $93,300.00

Salary is based on related experience, expertise, and internal equity; generally, new hires can expect pay between the minimum and midpoint of the range.

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