Isolation of DNA and RNA; construction and screening of cDNA libraries; PCR; RT-PCR; quantitative RT-PCR; Southern and Northern blotting; automated DNA sequencing; expression of plant genes in heterologous expression systems; transgenics
Recombinant protein expression and purification; SDS-PAGE; immunoblotting
Light, fluorescence and confocal microscopy; transmission electron microscopy; in situ localisation of macromolecules