The Agar group characterizes post-translational modifications of proteins and changes in protein, peptide, and lipid expression that occur during ALS, and then determines which of these changes have structural or toxic consequences. Because ALS begins with the death of a single type of cell, the motor neuron, we have developed numerous methods for the analysis of single cells by mass spectrometry. The Agar group specializes in mass spectrometry, including ultra-high mass resolution “top-down” mass spectrometry and mass spectrometry imaging methods, and has developed both analytical methods (“Matrix solution fixation” introduced by Agar YR 2007, automated funnel-skimmer dissociation, Karabacak 2008 and Cobb 2010, single-cell mass spectrometry imaging of mammalian cells, Agar YR 2010 and Boggio 2011) and computational tools (Karabacak 2008 introduced “Big Mascot” or Mascot TD database search engine, Li 2008 and 2010 introduce isotope calculator, a suite for mass spectrometry imaging applications is functional and to-be-submitted)
